Application

Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cell lysate (Santaguida, S., et al. (2011). EMBO J. 30(8):1508-1519.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Mad2 in PtK1 cells transfected with Alexa-Mad2 (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Mad2 in HeLa cells transfected with pCMV expressing wild-type myc-tagged Mad2, and certain mutations of myc-tagged Mad2 (This mutant does not bind to the core of Mad1/Mad2) (De Antoni, A., et al. 2005. Curr Biol. 15(3):214-225.).

Research CategoryEpigenetics & Nuclear Function

Anti-Mad2 Antibody, clone AS55-A12 is a highly specific mouse monoclonal antibody, that targets MAD2 & has been tested in western blotting & Immunofluorescence.

Research Sub CategoryCell Cycle, DNA Replication & Repair

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mad2, also known as MAD2, MAD2L1, or Mitotic spindle assembly checkpoint protein MAD2A, is a mitotic spindle assembly checkpoint protein. The spindle checkpoint makes sure that all the chromosomes are aligned at the metaphase plate before anaphase begins which means that all chromosomes are attached to the spindle before anaphase can start. The spindle checkpoint becomes triggered when the chromosome(s) are unaligned. Mad2 then binds to the anaphase promoting complex (APC) and CDC20. This complex prevents the cell from transitioning into anaphase and ultimately from cell death or disease.

Immunogen

His-tagged recombinant protein corresponding to human Mad2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Mad2 in 10 µg of HEK293 cell lyate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~23 kDa observed